Journal: Nature immunology

Article Title: Paired immunoglobulin-like receptor A is an intrinsic, self-limiting suppressor of IL-5-induced eosinophil development

doi: 10.1038/ni.2757

Figure Lengend Snippet: The expression of PIR-A throughout the low-density bone marrow (LDBM)-derived eosinophil culture was determined at the indicated time points (a). In (b) Pearson correlation between PIR-A and Bim expression is shown (r=0.943, P = 0.0004). LDBM cells from wild type (WT) and Pirb −/− mice were obtained and eosinophils were grown untreated (UT), in the presence of anti-PIR-A/B, or isotype control antibodies (Iso). Representative dot plots (c) and a summary of the flow cytometric analysis of anti-Siglec-F and annexin-V stained LDBM cells is shown (d, e). Cells were stained with modified Wright Giemsa stain and cellular morphology was assessed (f). Viable (i.e. Siglec-F + Annexin-V − ) Pirb −/− cells were sorted and PIR-A immunoprecipitated. Thereafter, antibody coated membranes were treated with anti-phospho-tyrosine antibodies conjugated to horseradish peroxidase and PIR-A:protein interactions were assessed (g). Representative flow cytometric histogram plot (h) and quantitative analysis of phopsho-Erk 1/2 expression in Siglec-F + Annexin-V − and Siglec-F + Annexin-V + cells from WT and Pirb −/− cells is shown (i). Expression of PIR-A and PIR-B on the surface of eosinophils from various in vivo sources is depicted (j); * P < 0.05, ** P < 0.01, *** P < 0.001. Data are representative dot-plots and mean and s.e.m of five experiments conducted in duplicates (a-f); representative blots of one out of three independent experiments (g); representative histogram plot and pooled mean and s.e.m from five mice; and mean and s.e.m of at least five mice.

Article Snippet: Custom-designed membranes coated with various antibodies were purchased from Hypromatrix.

Techniques: Expressing, Derivative Assay, Control, Staining, Modification, Giemsa Stain, Immunoprecipitation, In Vivo

Journal: Genomics Data

Article Title: Transcriptional profiling of CRP-regulated genes in deep-sea bacterium Shewanella piezotolerans WP3

doi: 10.1016/j.gdata.2015.04.019

Figure Lengend Snippet: Bacterial strains used in microarray study.

Article Snippet: Sequencer or array type , CapitalBio custom designed S. piezotolerans WP3 genome array.

Techniques: Microarray, Mutagenesis, Binding Assay

Journal: Genomics Data

Article Title: Transcriptional profiling of CRP-regulated genes in deep-sea bacterium Shewanella piezotolerans WP3

doi: 10.1016/j.gdata.2015.04.019

Figure Lengend Snippet: (A) Growth curve of WP3ΔcrpD1 and WP3ΔcrpD2 at 20 °C. The assays were performed in 2216E medium and oligotrophic medium, respectively. The average values and standard deviations displayed by the error bars resulted from three replicates. (B) Electrophoresis of total RNA of WP3 wild-type strain and crp gene mutants. Lane 1: WP3, lane 2: WP3ΔcrpD1, and lane 3: WP3ΔcrpD2.

Article Snippet: Sequencer or array type , CapitalBio custom designed S. piezotolerans WP3 genome array.

Techniques: Electrophoresis

Journal: Genomics Data

Article Title: Transcriptional profiling of CRP-regulated genes in deep-sea bacterium Shewanella piezotolerans WP3

doi: 10.1016/j.gdata.2015.04.019

Figure Lengend Snippet: Overview of gene expression by compares WP3-WT with WP3ΔcrpD1 (A) and WP3ΔcrpD2 (B) in the present experiment. X and Y axes present the intensity of gene transcription in WP3-WT and crp gene mutants, respectively. The black dots indicate genes with no significant change of transcriptional level, while red and green dots indicate up-regulated and down-regulated genes, respectively.

Article Snippet: Sequencer or array type , CapitalBio custom designed S. piezotolerans WP3 genome array.

Techniques: Gene Expression